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Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia

Received: 28 February 2017     Accepted: 19 April 2017     Published: 3 June 2017
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Abstract

The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity.

Published in International Journal of Science, Technology and Society (Volume 5, Issue 4)
DOI 10.11648/j.ijsts.20170504.12
Page(s) 62-66
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

in-vitro, FT-IR, Gas Chromatography–Mass Spectrometry (GC-MS), Antimicrobial Activity, Essential Oils, Belpharis linariifolia (Seeds)

References
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[2] Parekh J and Chanda S. In vitro antimicrobial activity of Trapa natans L. fruit rind extracted in different solvents. African Journal of Biotechnology, 6 (6), 2007, 760-770.
[3] Rinoldi MG. Problems in the diagnosis of invasive fungaldiseases. Review of infectious diseases, 13, 1991, 493-495.
[4] Meng JH, Zhao SH, Doyle MP, Joseph SW. Antibioticresistance of Escherichia coli O157: H7 and O157: NM isolatedfrom animals, food and humans. Journal of Food Protection, 61, 1998, 1511-1514.
[5] Perreten V, Giampa N, Schuler-Schmid U, Teuber M. Antibiotic resistance genes in coagulase-negativestaphylococci isolated from food. Systematic and AppliedMicrobiology, 21, 1998, 113-120.
[6] Kallio, H.; Kerrola, K.; Alhonmaki, P. Carvone and limonene in caraway fruits (Carum carvi L.) analyzed by supercritical carbon dioxide extraction-gas chromatography. J. Agric. Food Chem.1994, 42, 2478-2485.
[7] Burkil. H. M. The Useful Plants of West Tropical Africa. Blepharis linariifolia. Royal Botanic Garden; Kew. (1985-2004). [updated 2016 March 02; cited 2016 February 12] Available from: http://tropical.theferns.info/viewtropical.php?id=Blepharis+linariifolia.
[8] Wamtinga Richard Sawadogo, Aline Meda, Charles Euloge Lamien and Martin Kiendrebeogo. Phenolic Content and Antioxidant Activity of Six Acanthaceae from Burkina Faso. Journal of Biological Sciences. 2006; 6(2): 249-252.
[9] National Committee for Clinical Laboratory Standards (NCCLS) (1999). Performance standards for antimicrobial susceptibility testing; ninth informational supplement. Wayne, Pensilvania document M100-S9, Vol. 19. No. 1.
[10] Silva Junior AA, Vizotto VJ, Giongi E, Macedo SG, Marques LF (1994). Plantas medicinais, caracterização e cultivo. EPAGRI. Bol. Técnico Florianópolis, pp. 68: 1-71.
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  • APA Style

    Noha Ali Ibrahim, Safa Khalid Musa, Shima Mohammed Yassin, Nosiba Hashim Abuniama, Sufyan Awadalkareem, et al. (2017). Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. International Journal of Science, Technology and Society, 5(4), 62-66. https://doi.org/10.11648/j.ijsts.20170504.12

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    ACS Style

    Noha Ali Ibrahim; Safa Khalid Musa; Shima Mohammed Yassin; Nosiba Hashim Abuniama; Sufyan Awadalkareem, et al. Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. Int. J. Sci. Technol. Soc. 2017, 5(4), 62-66. doi: 10.11648/j.ijsts.20170504.12

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    AMA Style

    Noha Ali Ibrahim, Safa Khalid Musa, Shima Mohammed Yassin, Nosiba Hashim Abuniama, Sufyan Awadalkareem, et al. Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia. Int J Sci Technol Soc. 2017;5(4):62-66. doi: 10.11648/j.ijsts.20170504.12

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  • @article{10.11648/j.ijsts.20170504.12,
      author = {Noha Ali Ibrahim and Safa Khalid Musa and Shima Mohammed Yassin and Nosiba Hashim Abuniama and Sufyan Awadalkareem and Alsiddig Osama and Mohamed N. Abdalaziz},
      title = {Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia},
      journal = {International Journal of Science, Technology and Society},
      volume = {5},
      number = {4},
      pages = {62-66},
      doi = {10.11648/j.ijsts.20170504.12},
      url = {https://doi.org/10.11648/j.ijsts.20170504.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijsts.20170504.12},
      abstract = {The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Chemical Composition and Antimicrobial Activity of Essential Oil of Belpharis linariifolia
    AU  - Noha Ali Ibrahim
    AU  - Safa Khalid Musa
    AU  - Shima Mohammed Yassin
    AU  - Nosiba Hashim Abuniama
    AU  - Sufyan Awadalkareem
    AU  - Alsiddig Osama
    AU  - Mohamed N. Abdalaziz
    Y1  - 2017/06/03
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijsts.20170504.12
    DO  - 10.11648/j.ijsts.20170504.12
    T2  - International Journal of Science, Technology and Society
    JF  - International Journal of Science, Technology and Society
    JO  - International Journal of Science, Technology and Society
    SP  - 62
    EP  - 66
    PB  - Science Publishing Group
    SN  - 2330-7420
    UR  - https://doi.org/10.11648/j.ijsts.20170504.12
    AB  - The study was aimed to investigate essential oil chemical composition and antimicrobial activities of essential oils extracted from seeds of Belpharis linariifolia. The oil was extracted according to the method described by Harborne (1984). and analyzed by Fourier Transform Infrared Spectrophotometer (FTIR) and Gas Chromatography Mass Spectrometry (GC/MS) techniques to determine the chemical composition of the volatile fraction and identify their chemo-types. The essential oil of Belpharis linariifolia seeds were tested against four standard bacterial species: two Gram-positive bacteria viz, Bacillus subtilis (NCTC 8236) and Staphylococcus aureus (ATCC 25923), two Gram-negative bacterial strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and fungal strains viz, Candida albicans (ATCC 7596) using the paper disc diffusion method. Twenty two components were identified in the essential oil of Belpharis linariifolia representing 82.87% of the total components, the major compounds were Acetic acid (11.60%), 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl (11.60%), 3-Cyano-2-Oxa-1-Ethoxyadamanane (13.09%), Ethyl 3-methyl-2-oxobutyrate (15.49%), Hexatriacontane (8.18%) and Dotriacontane (16.03%). Antimicrobial activity of essential oil of Belpharis linariifolia dissolved in methanol (1:10), showed low activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (14 & 11 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (11.5 & 14 mm) and against (C. albicans) (zero mm). This study conducted for essential oil of Belpharis linariifolia seeds presence of variable compounds with diverse structures and low antimicrobial activity.
    VL  - 5
    IS  - 4
    ER  - 

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Author Information
  • Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

  • Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

  • Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

  • Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

  • Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

  • Chemistry Department, Omdurman Islamic University, Khartoum, Sudan

  • Medical Biochemistry Research Department, Medicinal and Aromatic Plants Research Institute, National Center for Research, Khartoum, Sudan

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